Journal: Molecular Vision

Article Title: Microarray reveals complement components are regulated in the serum-deprived rat retinal ganglion cell line

doi:

Figure Lengend Snippet: Genes identified in our microarray data and other.

Article Snippet: To identify potential signaling and pathways and gene-regulatory networks that are associated with retinal ganglion cell death,we imported the microarray expression data to PathwayAssist (Iobion, Informatics, LLC).

Techniques: Microarray, Membrane

Journal: Molecular Vision

Article Title: Microarray reveals complement components are regulated in the serum-deprived rat retinal ganglion cell line

doi:

Figure Lengend Snippet: Genes identified in our microarray and compared with other data.

Article Snippet: To identify potential signaling and pathways and gene-regulatory networks that are associated with retinal ganglion cell death,we imported the microarray expression data to PathwayAssist (Iobion, Informatics, LLC).

Techniques: Microarray, Binding Assay

Journal: Molecular Vision

Article Title: Microarray reveals complement components are regulated in the serum-deprived rat retinal ganglion cell line

doi:

Figure Lengend Snippet: Schematic representations of potential signaling pathways involved in serum-deprived retinal ganglion cell-5 cells. Pathways were identified by incorporating the microarray results (differentially expressed genes identified at 8, 24, 48, and 96 h of serum deprivation) into PathwayAssist software. The pathway connects the proteins (blue color) and cell processes together. Three major biological processes of cell survival, apoptosis, and DNA fragmentation, regulated by these genes are represented by yellow color squares.

Article Snippet: To identify potential signaling and pathways and gene-regulatory networks that are associated with retinal ganglion cell death,we imported the microarray expression data to PathwayAssist (Iobion, Informatics, LLC).

Techniques: Protein-Protein interactions, Microarray, Software

Journal: Molecular Vision

Article Title: Microarray reveals complement components are regulated in the serum-deprived rat retinal ganglion cell line

doi:

Figure Lengend Snippet: The biological pathway of the differentially expressed genes at 24, 48, and 96 h serum deprivation. A : Genes downregulated by serum deprivation (blue color). B : Genes upregulated by serum deprivation (violet color). The pathway was constructed on PathwayAssist software by searching for the shortest path to connect the genes of interest by other genes or cell processes with which they interacted through expression or regulation only. Each of these additional nodes has documented relation to apoptosis. The genes highlighted in blue color are the genes identified by microarray analysis, and the genes in red ovals diamonds color are the potential target genes identified with the aid of PathwayAssist.

Article Snippet: To identify potential signaling and pathways and gene-regulatory networks that are associated with retinal ganglion cell death,we imported the microarray expression data to PathwayAssist (Iobion, Informatics, LLC).

Techniques: Construct, Software, Expressing, Microarray

Journal: Molecular Vision

Article Title: Microarray reveals complement components are regulated in the serum-deprived rat retinal ganglion cell line

doi:

Figure Lengend Snippet: Biological pathway for the differentially expressed neuronal cell death genes in serum-deprived retinal ganglion cells. Pathways were identified by incorporating the microarray results (genes which are differentially expressed at 24, 48, and 96 h) into the Pathway Assit software. The pathway was constructed on this software by searching for the shortest path to connect the genes of interest by other genes or cell processes with which they interacted through expression or regulation only. Three major biologic processes are identified (apoptosis, death, and DNA fragmentation) and are represented by yellow rectangles. Blue ovals denote genes identified as neuronal cell death, and red ovals new genes connected to this pathway.

Article Snippet: To identify potential signaling and pathways and gene-regulatory networks that are associated with retinal ganglion cell death,we imported the microarray expression data to PathwayAssist (Iobion, Informatics, LLC).

Techniques: Microarray, Software, Construct, Expressing

Journal: Molecular Vision

Article Title: Microarray reveals complement components are regulated in the serum-deprived rat retinal ganglion cell line

doi:

Figure Lengend Snippet: Comparative evaluation of microarray and real-time RT-PCR results. Twelve genes, identified as differentially expressed in gene arrays and regulated in response to time-dependent serum deprivation, were evaluated with RT-PCR. Values on the y-axis represent the fold change derived from the mean expression value for each gene, and values on the x-axis represent the time course of RGC-5 serum deprivation. Total RNA isolated from these cells was used for both microarray analysis and real-time RT-PCR. The closed circles (rectangle) represent microarray results, whereas the closed squares (circle) represent the quantitative RT-PCR data.

Article Snippet: To identify potential signaling and pathways and gene-regulatory networks that are associated with retinal ganglion cell death,we imported the microarray expression data to PathwayAssist (Iobion, Informatics, LLC).

Techniques: Microarray, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Expressing, Isolation